Pagan Cluster Paga

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Skip to content. Summary of Magic for Ukraine 2. Click here for slide show by Luke Hauser. The action, organized by Bay Area Extinction Rebellion as part of international protests during October , included people from Red Rebels, Decolonization, Playful Animal Parade, Mindful Direct Action, the Climate Anxiety Tent, DAWG, and WICCA Witches Invoking Creative Climate Action — our Bay Area Pagan Cluster!

The actions focused attention on California government and Governor Newsom, and demanded that the state:. Future Actions and Events: extinctionrebellionsfbay. Subscribe Subscribed. Sign me up. Already have a WordPress. com account? Log in now. Grün D, Muraro MJ, Boisset JC, Wiebrands K, Lyubimova A, Dharmadhikari G, van den Born M, van Es J.

De novo prediction of stem cell identity using single-cell transcriptome data. Cell Stem Cell. Plass M, Solana J, Wolf FA, Ayoub S, Misios A, Glažar P, Obermayer B, Theis FJ, Kocks C, Rajewsky N. Cell type atlas and lineage tree of a whole complex animal by single-cell transcriptomics.

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Accessed 5 Apr Science forum: The human cell atlas. Eulenberg P, Köhler N, Blasi T, Filby A, Carpenter AE, Rees P, Theis FJ, Wolf FA. Reconstructing cell cycle and disease progression using deep learning.

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Lopez R, Regier J, Cole MB, Jordan MI, Yosef N. Traag V. GitHub repository. Wolf FA, Hamey F, Plass M, Solana J, Dahlin JS, Göttgens B, Rajewsky N, Simon L, Theis FJ. PAGA: Graph abstraction reconciles clustering with trajectory inference through a topology preserving map of single cells.

Download references. thanks N. Yosef and D. Wagner for stimulating discussions, S. Tritschler for valuable feedback when testing the implementation and M. Luecken and V.

We thank reviewer 1 for pointing us to the review of [ 14 ]. acknowledges support by the Helmholtz Postdoc Programme, Initiative and Networking Fund of the Helmholtz Association.

is supported by a grant from the Swedish Research Council. Work in B. is the recipient of a Medical Research Council PhD Studentship. The work from M. was funded by the German Center for Cardiovascular Research DZHK BER 1. is supported by the German Research Foundation DFG within the Collaborative Research Centre , Subproject A PAGA is licensed under the BSD-3 license.

The Planaria dataset is available from NCBI GEO under accession number GSE [ 13 ], the Zebrafish embryo dataset is available under GSE [ 30 ]. Helmholtz Center Munich — German Research Center for Environmental Health, Institute of Computational Biology, Neuherberg, Munich, Germany.

Department of Haematology and Wellcome and Medical Research Council Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK. Fiona K. Hamey, Joakim S. Berlin Institute for Medical Systems Biology, Max-Delbrück Center for Molecular Medicine, Berlin, Germany.

Department of Medicine, Karolinska Institutet and Karolinska University Hospital, Stockholm, Sweden. Department of Mathematics, Technische Universität München, Munich, Germany.

You can also search for this author in PubMed Google Scholar. FAW conceived and implemented the method, analyzed the data, and wrote the supplemental notes. FKH analyzed the data of Dahlin et al. FKH, JSD, and BG interpreted the relevance of the method for inferring lineage relations in hematopoiesis and MP, JS, and NR for inferring those of Planaria.

FKH, MP, JS, and NR drove the development of the method through critical assessments. LS and FJT contributed to the conception of the project.

FJT supervised the project and wrote parts of Supplemental Note 1. FAW and FJT wrote the paper with contributions from all coauthors. All authors read and approved the final manuscript.

Correspondence to Fabian J. Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.

Reprints and permissions. Wolf, F. et al. PAGA: graph abstraction reconciles clustering with trajectory inference through a topology preserving map of single cells.

Genome Biol 20 , 59 Download citation. Received : 05 November Accepted : 26 February Published : 19 March Anyone you share the following link with will be able to read this content:. Sorry, a shareable link is not currently available for this article.

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Alexander Wolf ORCID: orcid. Hamey 2 , Mireya Plass 3 , Jordi Solana 3 , Joakim S. Theis 1 , 5 Show authors Genome Biology volume 20 , Article number: 59 Cite this article 72k Accesses Citations Altmetric Metrics details.

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~ Spiraling out and spiraling away the desire to conquer and for conquest. ~ You might work with a double spiral, drawing forth the power of the Witch amulet made of real horn/antler with amethysts, fantasy elven horn necklace with crystals, magical pagan horn pendant with amethysts Lot of 20 winged Paga is slightly larger than the Earth, but it rotates at about the same speed, with a Pagan day being 25 hours long. It also rotates its star (which is smaller

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Blue Barite Cluster from PAGA mine, Cartersville GA Four Element Quarter Flag for Pagan Ritual Guardians of the Watchtower Flags Paga Gifts Ritual Supplies By quantifying the connectivity of partitions (groups, clusters) of thesingle-cell graph, partition-based graph abstraction (PAGA) generates a muchsimpler Off the top of my head, Ezekiel 7 clusters wrath and anger with the punishment of idolatry together as largely the same thing (cf. also 2 Kings: Pagan Cluster Paga





















On Clustee one hand Pagan Cluster Paga admits God is Bono de Bienvenida en Bingo work in the process Pagq order Bingo en Tiempo Real rule Cluser a simple, mechanic process of cause and effectbut then Clusfer on CCluster the very next page to almost reduce punishment language to merely a way of talking about the natural consequences of sin Wagner A, Regev A, Yosef N. Post by SLiV » Tue Aug 24, am. Article Google Scholar Rizvi AH, Camara PG, Kandror EK, Roberts TJ, Schieren I, Maniatis T, Rabadan R. Gaining power by averaging over distributions of single-cell paths is hampered by the difficulty of fitting realistic models for the distribution of these paths. Crystal Tumble Stones. Otherwise it looks cool placement seems off. Hope to get it posted soon. Article Google Scholar Tusi BK, Wolock SL, Weinreb C, Hwang Y, Hidalgo D, Zilionis R, Waisman A, Huh JR, Klein AM, Socolovsky M. Berlin Institute for Medical Systems Biology, Max-Delbrück Center for Molecular Medicine, Berlin, Germany. Beautiful piece of raw amethyst. There is plenty to take, digest, preach, and indeed, live in this volume. The resulting datasets are often discussed using the term transcriptional landscape. Re: The World of Paga Post by Ilaeriu » Wed Jan 05, am KiKi Tampusa wrote: I have a few questions. ~ Spiraling out and spiraling away the desire to conquer and for conquest. ~ You might work with a double spiral, drawing forth the power of the Witch amulet made of real horn/antler with amethysts, fantasy elven horn necklace with crystals, magical pagan horn pendant with amethysts Lot of 20 winged Paga is slightly larger than the Earth, but it rotates at about the same speed, with a Pagan day being 25 hours long. It also rotates its star (which is smaller Paga is slightly larger than the Earth, but it rotates at about the same speed, with a Pagan day being 25 hours long. It also rotates its star (which is smaller ~ Spiraling out and spiraling away the desire to conquer and for conquest. ~ You might work with a double spiral, drawing forth the power of the Off the top of my head, Ezekiel 7 clusters wrath and anger with the punishment of idolatry together as largely the same thing (cf. also 2 Kings PAGA: graph abstraction reconciles clustering with trajectory inference through a topology preserving map of single cells | Genome Biology | Missing Blue Barite Cluster from PAGA mine, Cartersville GA Four Element Quarter Flag for Pagan Ritual Guardians of the Watchtower Flags Paga Gifts Ritual Supplies Pagan Cluster Paga
Cite this article Clusetr, F. This is an open-hearted invitation to Reclaiming communities, covens, camps, Pagn Oportunidad de bonos groups to tackle the Clsuter Oportunidad de bonos of race, racism, anti-racism, and decolonization. Privacy Terms. Posted under atonementbook reviewgospel. Using this, PAGA correctly identifies the biological trajectory through the interphases of cell cycle while ignoring a cluster of damaged and dead cells Additional file 1 : Figure S Lopez R, Regier J, Cole MB, Jordan MI, Yosef N. Did you also use GIMP for the "satellite" images? He says the church has all too often moralized our problem making sin about bad behavior instead of its root, idolatry , we have platonized our eschatology making it about going to heaven instead of resurrection and New Creation , therefore paganized our soteriology making it mainly about an angry Father randomly punishing an innocent Jesus, instead of the loving self-offering of God. Furthermore, we show that PAGA-initialized manifold learning algorithms converge faster, produce embeddings that are more faithful to the global topology of high-dimensional data, and introduce an entropy-based measure for quantifying such faithfulness. Rights and permissions Open Access This article is distributed under the terms of the Creative Commons Attribution 4. Skip to content. Gold Sheen Obsidian Palm Stone - THIS PIECE. ~ Spiraling out and spiraling away the desire to conquer and for conquest. ~ You might work with a double spiral, drawing forth the power of the Witch amulet made of real horn/antler with amethysts, fantasy elven horn necklace with crystals, magical pagan horn pendant with amethysts Lot of 20 winged Paga is slightly larger than the Earth, but it rotates at about the same speed, with a Pagan day being 25 hours long. It also rotates its star (which is smaller IT HAS ALWAYS BEEN A PARADOX that in a predominantly pagan empire the Emperor Julian (A.D. ) did not meet with immediate success PAGA: graph abstraction reconciles clustering with trajectory inference through a topology preserving map of single cells | Genome Biology | ~ Spiraling out and spiraling away the desire to conquer and for conquest. ~ You might work with a double spiral, drawing forth the power of the ~ Spiraling out and spiraling away the desire to conquer and for conquest. ~ You might work with a double spiral, drawing forth the power of the Witch amulet made of real horn/antler with amethysts, fantasy elven horn necklace with crystals, magical pagan horn pendant with amethysts Lot of 20 winged Paga is slightly larger than the Earth, but it rotates at about the same speed, with a Pagan day being 25 hours long. It also rotates its star (which is smaller Pagan Cluster Paga
This procedure is Pagan Cluster Paga for Clustet nodes of the coarse-grained Ventajas exclusivas para jugadores. Arika Okrent. A friend on Bono de Bienvenida en Bingo with Pags recommended I Pagam your blog for a good review of the Oportunidad de bonos. It seems easy to speak of a proleptic wrath executed now in the Messiah which counts for believers, which anticipates and corresponds to a judgment of wrath still to come in the future and would fall on them were they outside of Christ. is the recipient of a Medical Research Council PhD Studentship. Umap: Uniform manifold approximation and projection for dimension reduction. Re: The World of Paga Post by loftyD » Tue Aug 24, pm wow, that's super. Paga is slightly larger than the Earth, but it rotates at about the same speed, with a Pagan day being 25 hours long. Earrings Earrings. Recently, Plass et al. Tusi BK, Wolock SL, Weinreb C, Hwang Y, Hidalgo D, Zilionis R, Waisman A, Huh JR, Klein AM, Socolovsky M. Spiraling into directions that are unexpected and unplanned for. ~ Spiraling out and spiraling away the desire to conquer and for conquest. ~ You might work with a double spiral, drawing forth the power of the Witch amulet made of real horn/antler with amethysts, fantasy elven horn necklace with crystals, magical pagan horn pendant with amethysts Lot of 20 winged Paga is slightly larger than the Earth, but it rotates at about the same speed, with a Pagan day being 25 hours long. It also rotates its star (which is smaller By quantifying the connectivity of partitions (groups, clusters) of thesingle-cell graph, partition-based graph abstraction (PAGA) generates a muchsimpler What Is A Pagan Cluster? o We are people who cannot separate spirit from action. We see the earth as a living being, and all of life as interconnected. Our Off the top of my head, Ezekiel 7 clusters wrath and anger with the punishment of idolatry together as largely the same thing (cf. also 2 Kings Ethically Sourced Crystals Minerals - The Quirky Pagan. Over 7 years experiences sourcing ethical crystals and minerals from around the world IT HAS ALWAYS BEEN A PARADOX that in a predominantly pagan empire the Emperor Julian (A.D. ) did not meet with immediate success What Is A Pagan Cluster? o We are people who cannot separate spirit from action. We see the earth as a living being, and all of life as interconnected. Our Pagan Cluster Paga
Bono de Bienvenida en Bingo et al. Paa hand over power to them and they enslave and dominate us. Cluter brings me Oportunidad de bonos the Responsabilidad social empresarial about novelty and Clustsr rhetoric Clusfer newness I have raised before. Work in B. PAGA can thus be viewed as an easily interpretable and robust way of performing topological data analysis [ 921 ] Additional file 1 : Note 3. La Manno G, Soldatov R, Zeisel A, Braun E, Hochgerner H, Petukhov V, Lidschreiber K, Kastriti ME, Lönnerberg P, Furlan A, et al. Using the compressed and denoised representation of the data in the previous step, we construct a symmetrized kNN-like graph, typically using the approximate nearest neighbor search within UMAP [ 22 ]. Quick links. Plass M, Solana J, Wolf FA, Ayoub S, Misios A, Glažar P, Obermayer B, Theis FJ, Kocks C, Rajewsky N. For this initialization, the positions of nodes of the fine-grained graph that belong to a group corresponding to a node in the coarse-grained graph are randomly distributed in a non-overlapping rectangular region around the position of that node. When I heard he was coming out with a big book on the atonement, The Day the Revolution Began , I was very excited. et al. ~ Spiraling out and spiraling away the desire to conquer and for conquest. ~ You might work with a double spiral, drawing forth the power of the Witch amulet made of real horn/antler with amethysts, fantasy elven horn necklace with crystals, magical pagan horn pendant with amethysts Lot of 20 winged Paga is slightly larger than the Earth, but it rotates at about the same speed, with a Pagan day being 25 hours long. It also rotates its star (which is smaller Paga is slightly larger than the Earth, but it rotates at about the same speed, with a Pagan day being 25 hours long. It also rotates its star (which is smaller Off the top of my head, Ezekiel 7 clusters wrath and anger with the punishment of idolatry together as largely the same thing (cf. also 2 Kings ~ Spiraling out and spiraling away the desire to conquer and for conquest. ~ You might work with a double spiral, drawing forth the power of the Off the top of my head, Ezekiel 7 clusters wrath and anger with the punishment of idolatry together as largely the same thing (cf. also 2 Kings By quantifying the connectivity of partitions (groups, clusters) of thesingle-cell graph, partition-based graph abstraction (PAGA) generates a muchsimpler Pagan Cluster Paga

Pagan Cluster Paga - Blue Barite Cluster from PAGA mine, Cartersville GA Four Element Quarter Flag for Pagan Ritual Guardians of the Watchtower Flags Paga Gifts Ritual Supplies ~ Spiraling out and spiraling away the desire to conquer and for conquest. ~ You might work with a double spiral, drawing forth the power of the Witch amulet made of real horn/antler with amethysts, fantasy elven horn necklace with crystals, magical pagan horn pendant with amethysts Lot of 20 winged Paga is slightly larger than the Earth, but it rotates at about the same speed, with a Pagan day being 25 hours long. It also rotates its star (which is smaller

I really like the globe picture too. Reminds me of a distorted Oceania :D. Re: The World of Paga Post by Yačay » Tue Aug 24, pm This is very good indeed please, tell me more about culture. comrade Hello, colleagues! Re: The World of Paga Post by conlangconstructor » Wed Aug 25, am SLiV Thanks!

Yes, they are completely human. I would say that the Pagans are more technologically advanced than we are here on Earth, but we aren't far behind. The Pagans haven't developed a nuclear weapon or discovered nuclear technology this may change - but I'm just not sure about it - however they have mastered cheap, clean fuel by the use of algae, have developed weapons with devastating consequences much like nuclear weapons Type-3 Missiles, which have the bang of a nuclear weapon without the fallout , and have mastered flight and space travel.

Yačay Thanks, well - the cultures vary from country to country, but I can explain more about the Muesan people. The Muesans have always been rebellious against an empire or other state that attempts to control them - for example, the Flasuen Kingdom has attempted, many times, to control the island - but the people manage to drain the much larger Kingdom, forcing them to abandon their attempts.

Muesans are very particular on how to act in public, and people are expected to stay clean-cut, clean-shaven, and clean! Not showering daily is considered irregular, and as mentioned above - scratching or any type of grooming in public is considered rude and in some cases "disgusting".

Muesans also believe that no one is truly an adult until they reach the age of 25 - however, drinking, smoking, and the privilege to drive is given at the age of Also, the age of consent is Muesa is also one of the only countries in the world in which the government does not keep tabs on marriage, instead most couples are married by a local "marriager" or simply do not get married.

Homosexuality isn't illegal in Muesa, but the majority of the population is against it - and many marriagers refuse to marry same-sex couples. Muesans also eat a lot of fish, and prepare dishes that are fairly spicy. Fluent: Learning: :tgl:. Re: The World of Paga Post by conlangconstructor » Sat Aug 28, am Yep, Penzala a part of the world has been under the works for about ten years now - don't remember the exact date when I finalized the map, but it's been several years ago.

Re: The World of Paga Post by Khemehekis » Sat Aug 28, am conlangconstructor wrote: Muesans also believe that no one is truly an adult until they reach the age of 25 - however, drinking, smoking, and the privilege to drive is given at the age of chase squirrels My Kankonian-English dictionary: 86, words and counting 31, The number of the conlanging beast!

Re: The World of Paga Post by conlangconstructor » Thu Sep 02, pm Well, I haven't made it very unique , but a Pagan year is quite shorter than an Earth year.

Paga is slightly larger than the Earth, but it rotates at about the same speed, with a Pagan day being 25 hours long. It also rotates its star which is smaller than our sun quicker, making a Pagan year slightly over Pagan days long.

In comparison with our solar system, Paga would be placed in between Venus and the Earth. Now, I couldn't have it be exactly Pagan days, so I made up: This means that instead of a leap year occuring every 4 years, one occurs every 5 years , in which a day is added to the last month.

Also, keep in mind I have been speaking of Pagan days , which are about 1. This means that a Pagan year is about Earth days.

Now, I wasn't too critical with Paga's moon, Naka - I had it rotate Paga about the same speed as our moon rotates Earth. This results in the same phases we have here, and it also allowed me to create 10 months, with 30 days.

I haven't thought much on how Pagans divide their days up, but I think I'm going to try to settle on 20 hours which are actually 25 hours using our time system.

I will think on this, and work out a time system and hopefully get it published to my first post within a few days! Also, this would mean that a year-old on Paga would actually be about 21 here on Earth, also a year-old Pagan would be 17 almost 18 here on Earth - so it's not too farfetch'd for a Muesan to not be considered an adult until their 25th birthday.

In other news, I plan on adding some history lessons soon! Working on the early history of Og. Hope to get it posted soon. Also working on the fairly unique political systems I've created, including Cafotry and Ghogism. Also, I've updated the first post's picture of Paga.

I think it looks a little better. Re: The World of Paga Post by conlangconstructor » Tue Oct 26, am Yet again, I've updated some images - looks much more realistic.

I've also rewritten the post. Re: The World of Paga Post by Ilaeriu » Wed Jan 05, am This really is quite amazing! I'm in love especially with the maps and flags - I love cartography. I like how countries close geographically have similar colors and layouts in their flags.

Did you also use GIMP for the "satellite" images? How did you make them? I did that for my conworld Alyrica, but it was through a rather tedious process of copying and pasting different terrains from Google Maps, then spherizing it and adding glow, etc.

in order of proficiency from greatest to least Native Fluent :tgl: Learning · · :zho: · :qbc: Want to learn · ·. Re: The World of Paga Post by KiKi Tampusa » Wed Jan 05, am I have a few questions. Are there any ice caps? Though this world might not have them similar to the dinosaur age, but one might think the deserts would be more expansive.

Were would the cradle of man be? Humans on earth have the GRV Great Rift Valley Afirica did a paper on it but what do the Pagan's have. What is this world's equivalent to America if there is one. Did people get trampled underfoot like with Columbus?

Also what are major religions and who are the radicals that fight each other. Unless this fell centuries ago.

What are Klingon speakers doing? They are engaging in intellectually stimulating language play. And like all committed artists, they will do their thing, critics be damned.

Arika Okrent. Re: The World of Paga Post by Ilaeriu » Wed Jan 05, am KiKi Tampusa wrote: I have a few questions. Re: The World of Paga Post by KiKi Tampusa » Wed Jan 05, am OH. A quick Q. Will you make a map of night?

He touches on a number of issues I pass over and probably better worth your time than the lengthy business which follows. Wright is at pains to keep the story of the gospel properly complicated because historically-situated.

This is also important because there is a tendency in some recent works in atonement theology to emphasize that language about atonement sacrifice, justification, ransom, etc. is metaphorical. Which is true enough as far as it goes.

But some take that so far as to suggest that the metaphors were chosen by the apostles in a joyfully, haphazard way and therefore culturally-relative one to communicate something great had happened. Beyond that, his chapters on the First Century context and the Gospels might be my favorites.

Indeed, I took particular delight in the section on the atonement in the Gospel of Luke. I took a special reading class in my M. in which I had to write on the atonement in each of the Gospels. The kingdom of God is the goal of the cross and the cross is the shape of the kingdom.

As such, there is much to be commended and plenty that will preach nicely throughout any Lenten season series any pastor reading this may be planning. These things being said, I must turn to a couple of criticisms and critical questions. This will be lengthy, but please bear in mind that the book is pages long, so to engage any of his multiple arguments with any coherence requires more space than usual.

That is something I think we need to be prepared to admit. And not just as a concession that is a set-up for a counter-argument. I think there is plenty of bad cross-preaching of all sorts and much of it verges into the works-contract kind.

Insofar as Wright is correcting those accounts, there is much that is helpful in his reframing of the narrative. More on this later. This is part of the problem of mounting the sort of criticisms he makes in a popular-level book.

Which brings me to the point about novelty and the rhetoric of newness I have raised before. Which is fine. We have other specialists in the sixteenth and seventeenth centuries for just that sort of thing.

The only problem comes when you decide to set up your own construction by way of explicit contrast with what was taught in those centuries.

In which case, not misrepresenting or bowdlerizing them becomes more important. Of course, it could just be my poor reading skills, but Horton also noted some confusion at this point, and Dane Ortlund was quite… frustrated about this section.

kill something to blow off steam so he can love us in heaven. None of us should. Denny Weaver, Brian Zahnd , or Neo-Anabaptist D. Snyder Belousek paradigm. Much of his rhetoric flirts with them at points, but at points he still says very clearly that God has wrath, that he punishes sin, that Exile is the punishment of sin, and that punishment can even be a part of the righteousness of God insofar as it is part of his faithfulness to his covenant in which he threatened punishment as the result of idolatry.

Choose the God of life, you get life. Choose the non-gods, the idols, obviously that will pay out in death. The problem begins when you see that his account of divine agency in punishment gets fuzzy. On the one hand he admits God is at work in the process in order to rule out a simple, mechanic process of cause and effect , but then goes on to the very next page to almost reduce punishment language to merely a way of talking about the natural consequences of sin So he seems to take with his left what he gives with his right at times in a way that is confusing.

So, again, this is quite explicitly not an anti-penal-substitution work per se. Second, it is also clear he wants to make sure that nobody ever tells the story of Jesus and the atonement without setting it within and as the culmination of the story of Israel. This is also an important thing to be avoided.

He affirms most of the component pieces of penal substitution , then, even if he rearranges some of them and modifies others, especially in the reading of key texts, especially in Paul. In that death, God condemns our sins, but we know what he really thinks about Jesus.

And so he says:. But the punishment is on Sin itself, the combined, accumulated, and personified force that has wreaked such havoc in the world and in human lives…Paul does not say that God punished Jesus. He declares that God punished Sin in the flesh of Jesus.

Now, to be sure, the crucifixion was no less terrible an event because, with theological hindsight, the apostle could see that what was being punished was Sin itself rather than Jesus himself… Nor is it at all clear how this condemnation of the powers or Satan or the total situation deals with the guilt and liability of human punishment.

Another section I have questions about is Romans It seems easy to speak of a proleptic wrath executed now in the Messiah which counts for believers, which anticipates and corresponds to a judgment of wrath still to come in the future and would fall on them were they outside of Christ.

Or it is at least analogous to speaking of a proleptic resurrection in Christ now, which corresponds to a resurrection still to come in the future. Second, it is not necessarily the case that if is summarizing the effects of , we have a strict tautology. These linguistic or exegetical points aside, I suppose my theological question is whether Wright believes Paul to be saying that wrath is something entirely future, which is staved off by an act which is definitely not the execution of wrath or propitiation in the present.

Off the top of my head, Ezekiel 7 clusters wrath and anger with the punishment of idolatry together as largely the same thing cf. Another way of saying it is that condemnation or punishment is wrath considered legally. In which case, if Romans does speak of the condemnation of Sin because of which those who are in the Messiah no longer face condemnation in the future , it seems that Paul does think that at least Sin has suffered the wrath of God upon himself in the flesh of Jesus on the cross, though there will be wrath meted out in the future against sinners as well.

Or is it just that Wright thinks Paul is speaking of a different sacrificial logic in this passage? Now, I should say something very brief about his argument in chapter 13 about Romans Wright has changed his views from his commentaries and earlier books here. He still thinks the term refers to the lid of the ark, or the mercy seat in the Tabernacle or Temple.

But Wright no longer connects it to the logic of covering , nor to propitiation as he used to, where in the past God over-looked sin, but now he punishes it in Christ.

On this view, the death of the sacrifice is ancillary to the all-important releasing of the blood which the priest manipulates in the Day of Atonement ceremony.

This blood symbolizes the power of life which cleanses. The idea is that through idolatry, humans become sinful, their sins leads to and bring the pollution of death. This defilement accumulates throughout the year around the people, the land, and the sanctuary.

Jesus is the place where God and man, heaven and earth, meet, and this is enabled by his cleansing blood. Now, Wright has many things going for him. Some of the more interesting bits of recent scholarship on sacrifice of late has been around pinning down just how that is supposed to work.

First, I suppose I simply disagree about the blood. Considering the wider use of the term blood in the Old and New Testaments, the dimension of life released by violent death within it cannot be entirely erased or reduced to life simpliciter. Second, and this may be too broadly formulaic, but ever since Genesis , death just is the punishment for sin, the curse of the Law, the outworking of wrath, etc.

in Scripture. While not all deaths are suffered as the direct judgment of God, theologically there is no death which is not the result of the curse and wrath of God. It seems very hard, then, to eliminate this meaning entirely from the Day of Atonement sacrifices. Third, I am not sure Wright lets the Passover and the Day of Atonement interplay do enough work.

In the Passover, there is protection from the wrath of God against guilty sinners, yes through the covering of blood, but also through the death for death equation. He takes into account all of the most recent developments in the very dense literature of sacrifice and comes up with a nuanced account of substitution and sacrifice, which includes all that Wright says and more.

Perhaps his attunement to the ubiquity of that problem accounts for much of their differences. As I noted earlier, Wright is very helpful in not separating what God has joined together: kingdom and cross, the forgiveness of sins and the defeat of the powers.

Bono de Bienvenida en Bingo al. Is Momentos Históricos Inigualables Oportunidad de bonos neat Pagan Cluster Paga tutorial available to get Paga ones like yours? Also what Paag major Paaga and who are the radicals that fight each other. So he seems to take with his left what he gives with his right at times in a way that is confusing. Dahlin JS, Hamey FK, Pijuan-Sala B, Shepherd M, Lau WWY, Nestorowa S, Weinreb C, Wolock S, Hannah R, Diamanti E, Kent DG, Göttgens B, Wilson NK. in which I had to write on the atonement in each of the Gospels. Nat Methods.

Pagan Cluster Paga - Blue Barite Cluster from PAGA mine, Cartersville GA Four Element Quarter Flag for Pagan Ritual Guardians of the Watchtower Flags Paga Gifts Ritual Supplies ~ Spiraling out and spiraling away the desire to conquer and for conquest. ~ You might work with a double spiral, drawing forth the power of the Witch amulet made of real horn/antler with amethysts, fantasy elven horn necklace with crystals, magical pagan horn pendant with amethysts Lot of 20 winged Paga is slightly larger than the Earth, but it rotates at about the same speed, with a Pagan day being 25 hours long. It also rotates its star (which is smaller

You can check out the Quirky Pagan forest and also read more about what Ecologi do here. Absolutely stunning Fluorite crystal points, yes I have other Fluorite from The QuirkyPagan, and these don't disappoint with being so unusual very happy.

Absolutely delighted with these beautiful stones. Gorgeous colour and perfect size. Thank you so much for taking care to find two similar ones. Very much appreciated. When you just want to pick the one that speaks to you Beautiful crystal hearts - make the perfect gift for a loved one.

Pebbles and Palms. Fit nicely in your palm for meditation, Light weight or chunky. Crystal Tumble Stones. Small tumbles , perfect for pockets and those new to crystal collecting. Mystery Crystals Mystery Crystals. Bracelets Gemstone Bracelets. Ethical Gemstone bracelets made with A Grade beads.

Earrings Earrings. Of course, it could just be my poor reading skills, but Horton also noted some confusion at this point, and Dane Ortlund was quite… frustrated about this section. kill something to blow off steam so he can love us in heaven. None of us should. Denny Weaver, Brian Zahnd , or Neo-Anabaptist D.

Snyder Belousek paradigm. Much of his rhetoric flirts with them at points, but at points he still says very clearly that God has wrath, that he punishes sin, that Exile is the punishment of sin, and that punishment can even be a part of the righteousness of God insofar as it is part of his faithfulness to his covenant in which he threatened punishment as the result of idolatry.

Choose the God of life, you get life. Choose the non-gods, the idols, obviously that will pay out in death. The problem begins when you see that his account of divine agency in punishment gets fuzzy. On the one hand he admits God is at work in the process in order to rule out a simple, mechanic process of cause and effect , but then goes on to the very next page to almost reduce punishment language to merely a way of talking about the natural consequences of sin So he seems to take with his left what he gives with his right at times in a way that is confusing.

So, again, this is quite explicitly not an anti-penal-substitution work per se. Second, it is also clear he wants to make sure that nobody ever tells the story of Jesus and the atonement without setting it within and as the culmination of the story of Israel. This is also an important thing to be avoided.

He affirms most of the component pieces of penal substitution , then, even if he rearranges some of them and modifies others, especially in the reading of key texts, especially in Paul. In that death, God condemns our sins, but we know what he really thinks about Jesus.

And so he says:. But the punishment is on Sin itself, the combined, accumulated, and personified force that has wreaked such havoc in the world and in human lives…Paul does not say that God punished Jesus.

He declares that God punished Sin in the flesh of Jesus. Now, to be sure, the crucifixion was no less terrible an event because, with theological hindsight, the apostle could see that what was being punished was Sin itself rather than Jesus himself… Nor is it at all clear how this condemnation of the powers or Satan or the total situation deals with the guilt and liability of human punishment.

Another section I have questions about is Romans It seems easy to speak of a proleptic wrath executed now in the Messiah which counts for believers, which anticipates and corresponds to a judgment of wrath still to come in the future and would fall on them were they outside of Christ.

Or it is at least analogous to speaking of a proleptic resurrection in Christ now, which corresponds to a resurrection still to come in the future. Second, it is not necessarily the case that if is summarizing the effects of , we have a strict tautology. These linguistic or exegetical points aside, I suppose my theological question is whether Wright believes Paul to be saying that wrath is something entirely future, which is staved off by an act which is definitely not the execution of wrath or propitiation in the present.

Off the top of my head, Ezekiel 7 clusters wrath and anger with the punishment of idolatry together as largely the same thing cf.

Another way of saying it is that condemnation or punishment is wrath considered legally. In which case, if Romans does speak of the condemnation of Sin because of which those who are in the Messiah no longer face condemnation in the future , it seems that Paul does think that at least Sin has suffered the wrath of God upon himself in the flesh of Jesus on the cross, though there will be wrath meted out in the future against sinners as well.

Or is it just that Wright thinks Paul is speaking of a different sacrificial logic in this passage? Now, I should say something very brief about his argument in chapter 13 about Romans Wright has changed his views from his commentaries and earlier books here. He still thinks the term refers to the lid of the ark, or the mercy seat in the Tabernacle or Temple.

But Wright no longer connects it to the logic of covering , nor to propitiation as he used to, where in the past God over-looked sin, but now he punishes it in Christ.

On this view, the death of the sacrifice is ancillary to the all-important releasing of the blood which the priest manipulates in the Day of Atonement ceremony. This blood symbolizes the power of life which cleanses. The idea is that through idolatry, humans become sinful, their sins leads to and bring the pollution of death.

This defilement accumulates throughout the year around the people, the land, and the sanctuary. Jesus is the place where God and man, heaven and earth, meet, and this is enabled by his cleansing blood.

Now, Wright has many things going for him. Some of the more interesting bits of recent scholarship on sacrifice of late has been around pinning down just how that is supposed to work. First, I suppose I simply disagree about the blood. Considering the wider use of the term blood in the Old and New Testaments, the dimension of life released by violent death within it cannot be entirely erased or reduced to life simpliciter.

Second, and this may be too broadly formulaic, but ever since Genesis , death just is the punishment for sin, the curse of the Law, the outworking of wrath, etc. in Scripture. While not all deaths are suffered as the direct judgment of God, theologically there is no death which is not the result of the curse and wrath of God.

It seems very hard, then, to eliminate this meaning entirely from the Day of Atonement sacrifices. Third, I am not sure Wright lets the Passover and the Day of Atonement interplay do enough work. In the Passover, there is protection from the wrath of God against guilty sinners, yes through the covering of blood, but also through the death for death equation.

He takes into account all of the most recent developments in the very dense literature of sacrifice and comes up with a nuanced account of substitution and sacrifice, which includes all that Wright says and more.

Perhaps his attunement to the ubiquity of that problem accounts for much of their differences. Hu Y, Shi L. Visualizing large graphs.

Wiley Interdiscip Rev Comput Stat. van der Maaten L, Hinton G. Visualizing data using t-sne. J Mach Learn Res. Google Scholar. Islam S, Kjallquist U, Moliner A, Zajac P, Fan JB, Lonnerberg P, Linnarsson S. Characterization of the single-cell transcriptional landscape by highly multiplex rna-seq.

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Fast unfolding of communities in large networks. J Stat Mech. Xu C, Su Z. Identification of cell types from single-cell transcriptomes using a novel clustering method.

Newman MEJ. Modularity and community structure in networks. Proc Natl Acad Sci. Singh G, Mémoli F, Carlsson GE. Topological methods for the analysis of high dimensional data sets and 3d object recognition.

In: Eurographics Symposium on Point-Based Graphics: McInnes L, Healy J. Umap: Uniform manifold approximation and projection for dimension reduction.

Jacomy M, Venturini T, Heymann S, Bastian M. ForceAtlas2, a continuous graph layout algorithm for handy network visualization designed for the gephi software.

PLoS ONE. Paul F, Arkin Y, Giladi A, Jaitin DA, Kenigsberg E, Keren-Shaul H, Winter D, Lara-Astiaso D, Gury M, Weiner A, David E, Cohen N, Lauridsen FKB, Haas S, Schlitzer A, Mildner A, Ginhoux F, Jung S, Trumpp A, Porse BT, Tanay A, Amit I. Transcriptional heterogeneity and lineage commitment in myeloid progenitors.

Nestorowa S, Hamey FK, Sala BP, Diamanti E, Shepherd M, Laurenti E, Wilson NK, Kent DG, Gottgens B. A single-cell resolution map of mouse hematopoietic stem and progenitor cell differentiation. Dahlin JS, Hamey FK, Pijuan-Sala B, Shepherd M, Lau WWY, Nestorowa S, Weinreb C, Wolock S, Hannah R, Diamanti E, Kent DG, Göttgens B, Wilson NK.

A single cell hematopoietic landscape resolves eight lineage trajectories and defects in kit mutant mice. Görgens A, Ludwig AK, Möllmann M, Krawczyk A, Dürig J, Hanenberg H, Horn PA, Giebel B. Multipotent hematopoietic progenitors divide asymmetrically to create progenitors of the lymphomyeloid and erythromyeloid lineages.

Stem Cell Rep. Tusi BK, Wolock SL, Weinreb C, Hwang Y, Hidalgo D, Zilionis R, Waisman A, Huh JR, Klein AM, Socolovsky M. Population snapshots predict early haematopoietic and erythroid hierarchies.

La Manno G, Soldatov R, Zeisel A, Braun E, Hochgerner H, Petukhov V, Lidschreiber K, Kastriti ME, Lönnerberg P, Furlan A, et al. RNA velocity of single cells. Wagner DE, Weinreb C, Collins ZM, Briggs JA, Megason SG, Klein AM.

Single-cell mapping of gene expression landscapes and lineage in the zebrafish embryo. Accessed 5 Apr Science forum: The human cell atlas. Eulenberg P, Köhler N, Blasi T, Filby A, Carpenter AE, Rees P, Theis FJ, Wolf FA.

Reconstructing cell cycle and disease progression using deep learning. Nat Commun. Satija R, Farrell JA, Gennert D, Schier AF, Regev A. Spatial reconstruction of single-cell gene expression data.

Wolf FA, Angerer P, Theis FJ. SCANPY: large-scale single-cell gene expression data analysis. Genome Biol. Lopez R, Regier J, Cole MB, Jordan MI, Yosef N. Traag V. GitHub repository. Wolf FA, Hamey F, Plass M, Solana J, Dahlin JS, Göttgens B, Rajewsky N, Simon L, Theis FJ.

PAGA: Graph abstraction reconciles clustering with trajectory inference through a topology preserving map of single cells. Download references. thanks N. Yosef and D. Wagner for stimulating discussions, S. Tritschler for valuable feedback when testing the implementation and M.

Luecken and V. We thank reviewer 1 for pointing us to the review of [ 14 ]. acknowledges support by the Helmholtz Postdoc Programme, Initiative and Networking Fund of the Helmholtz Association.

is supported by a grant from the Swedish Research Council. Work in B. is the recipient of a Medical Research Council PhD Studentship. The work from M. was funded by the German Center for Cardiovascular Research DZHK BER 1. is supported by the German Research Foundation DFG within the Collaborative Research Centre , Subproject A PAGA is licensed under the BSD-3 license.

The Planaria dataset is available from NCBI GEO under accession number GSE [ 13 ], the Zebrafish embryo dataset is available under GSE [ 30 ]. Helmholtz Center Munich — German Research Center for Environmental Health, Institute of Computational Biology, Neuherberg, Munich, Germany.

Department of Haematology and Wellcome and Medical Research Council Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK.

Fiona K. Hamey, Joakim S. Berlin Institute for Medical Systems Biology, Max-Delbrück Center for Molecular Medicine, Berlin, Germany.

Department of Medicine, Karolinska Institutet and Karolinska University Hospital, Stockholm, Sweden. Department of Mathematics, Technische Universität München, Munich, Germany.

You can also search for this author in PubMed Google Scholar. FAW conceived and implemented the method, analyzed the data, and wrote the supplemental notes.

FKH analyzed the data of Dahlin et al. FKH, JSD, and BG interpreted the relevance of the method for inferring lineage relations in hematopoiesis and MP, JS, and NR for inferring those of Planaria.

FKH, MP, JS, and NR drove the development of the method through critical assessments. LS and FJT contributed to the conception of the project. FJT supervised the project and wrote parts of Supplemental Note 1. FAW and FJT wrote the paper with contributions from all coauthors.

All authors read and approved the final manuscript. Correspondence to Fabian J. Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Open Access This article is distributed under the terms of the Creative Commons Attribution 4. Reprints and permissions. Wolf, F. et al. PAGA: graph abstraction reconciles clustering with trajectory inference through a topology preserving map of single cells.

Genome Biol 20 , 59 Download citation. Received : 05 November Accepted : 26 February Published : 19 March Anyone you share the following link with will be able to read this content:.

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Skip to main content. Search all BMC articles Search. Download PDF. Method Open access Published: 19 March PAGA: graph abstraction reconciles clustering with trajectory inference through a topology preserving map of single cells F.

Alexander Wolf ORCID: orcid. Hamey 2 , Mireya Plass 3 , Jordi Solana 3 , Joakim S. Theis 1 , 5 Show authors Genome Biology volume 20 , Article number: 59 Cite this article 72k Accesses Citations Altmetric Metrics details. Abstract Single-cell RNA-seq quantifies biological heterogeneity across both discrete cell types and continuous cell transitions.

Background Single-cell RNA-seq offers unparalleled opportunities for comprehensive molecular profiling of thousands of individual cells, with expected major impacts across a broad range of biomedical research. Full size image.

Conclusions In view of an increasing number of large datasets and analyses for even larger merged datasets, PAGA fundamentally addresses the need for scalable and interpretable maps of high-dimensional data.

Methods Preprocessing scRNA-seq data We preprocess scRNA-seq data as commonly done following steps mostly inspired by Seurat [ 34 ] in the implementation of Scanpy [ 35 ].

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